colorante primario 18
PRIMARY DYE To study microorganisms, Gram staining is used. Dyes are substances that have the ability to stain cells, structures or tissues. According to their origin, they allow to make microscopic and transparent objects visible, to know their shape and size, as well as their internal and external structures. Crystal violet is the best primary dye for a Gram stain. The color difference is related to the type of bacterial wall: violet-colored cells (Gram-positive wall bacteria) and pink cells (Gram-negative wall bacteria). Safranin is a secondary or contratinction dye that stains bacteria that could not retain the violet-iodine crystal complex.